General Information

Product

TLR3 Antibody

Description

Affinity Purified Toll-like receptor 3 Antibody

Verified Applications

ELISA, IHC,IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 650-700 on human TLR3 protein.

Accession #

GenBank: AAH96333.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.65-0.77 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:20,000

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

O15455

Overview

Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific to microorganisms. TLR3 is a nucleotide-sensing TLR which is activated by double-stranded RNA, a sign of viral infection.

Molecular Function

Receptor, double-stranded RNA binding, transmembrane signaling receptor activity

Subcellular Location

Endoplasmic reticulum membrane; Single-pass type I membrane protein, Endosome membrane

Expression

Expressed at high level in placenta and pancreas. Also detected in CD11c+ immature dendritic cells. Only expressed in dendritic cells and not in other leukocytes, including monocyte precursors. TLR3 is the TLR that is expressed most strongly in the brain, especially in astrocytes, glia, and neurons.

Structure

Monomer and homodimer; dimerization is triggered by ligand-binding, the signaling unit is composed of one ds-RNA of around 40 bp and two TLR3 molecules, and lateral clustering of signaling units along the length of the ds-RNA ligand is required for TLR3 signal transduction. Interacts (via transmembrane domain) with UNC93B1; the interaction is required for transport from the ER to the endosomes.

Alternative Nomenclature

CD283 antibody

CD283 antigen antibody

IIAE2 antibody

TLR 3 antibody

Toll Like Receptor 3 antibody