General Information

Product

DAPK2 Antibody

Description

Affinity Purified Death-associated protein kinase 2 Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Mouse, Rat

Immunogen

Synthetic peptide take within amino acid region 300-350 on mouse Death-associated protein kinase 2 protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5-1.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Purification Method

Immobilized antigen affinity chromatography

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:50,000

Immunoprecipitation

1:200

Western Blot

1:2,500-1:5,000

Protein

Uniprot #

Q8VDF3

Overview

Calcium/calmodulin-dependent serine/threonine kinase involved in multiple cellular signaling pathways that trigger cell survival, apoptosis, and autophagy. Capable of regulating both type I apoptotic and type II autophagic cell death signals.

Molecular Function

ATP binding

calmodulin binding

identical protein binding

protein serine/threonine kinase activity

Subcellular Location

Cytoplasm

autophagosome lumen

Expression

Expressed in peritubular interstitial cells of the renal cortex (PubMed:24906443). Isoform 1 is found in the adult brain while isoform 2 is expressed in brains of embryos and young mice (at protein level) (PubMed:21408167).

Structure

The autoinhibitory domain sterically blocks the substrate peptide-binding site by making both hydrophobic and electrostatic contacts with the kinase core.

Alternative Nomenclature

DAK2 antibody

DAP kinase 2 antibody

DAP kinase related protein 1 antibody

DAP kinase related protein 1 beta isoform antibody

DAP-kinase-related protein 1 antibody

DAPK2 antibody

Death associated protein kinase 2 antibody

DRP 1 antibody