General Information

Product

HMGB1 Antibody

Description

Affinity Purified High mobility group protein B1 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 150-200 on human High mobility group protein B1 protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5-1.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Purification Method

Immobilized antigen affinity chromatography

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:50,000

Immunoprecipitation

1:200

Western Blot

1:2,500-1:5,000

Protein

Uniprot #

P09429

Overview

Multifunctional redox sensitive protein with various roles in different cellular compartments. In the nucleus is one of the major chromatin-associated non-histone proteins and acts as a DNA chaperone involved in replication, transcription, chromatin remodeling, V(D)J recombination, DNA repair and genome stability. Proposed to be an universal biosensor for nucleic acids.

Molecular Function

bubble DNA binding

chemoattractant activity

C-X-C chemokine binding

cytokine activity

damaged DNA binding

DNA binding, bending

DNA binding transcription factor activity

DNA polymerase binding

double-stranded DNA binding

four-way junction DNA binding

integrin binding

lipopolysaccharide binding

lyase activity

phosphatidylserine binding

RAGE receptor binding

repressing transcription factor binding

RNA binding

single-stranded DNA binding

supercoiled DNA binding

transcription factor binding

Subcellular Location

Extracellular region or secreted, nucleus, endosome, plasma membrane, chromosome, cytoplasm, endoplasmic reticulum-golgi intermediate compartment

Expression

Ubiquituous. Expressed in platelets.

Structure

Belongs to the HMGB family.

Alternative Nomenclature

Amphoterin antibody

Chromosomal protein, nonhistone, HMG1 antibody

High mobility group box 1 antibody

High mobility group protein 1 antibody

High mobility group protein B1 antibody

high-mobility group (nonhistone chromosomal) protein 1 antibody

HMG-1 antibody

HMG3 antibody

HMGB 1 antibody

NONHISTONE CHROMOSOMAL PROTEIN HMG1 antibody

SBP 1 antibody

Sulfoglucuronyl carbohydrate binding protein antibody