General Information

Product

HIPK3 Antibody

Description

Affinity Purified Homeodomain-interacting protein kinase 3 Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino aci region 860-910 on human Homeodomain-interacting protein kinase 3 protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5-1.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Purification Method

Immobilized antigen affinity chromatography

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:50,000

Immunoprecipitation

1:200

Western Blot

1:2,500-1:5,000

Protein

Uniprot #

Q9H422

Overview

Serine/threonine-protein kinase involved in transcription regulation, apoptosis and steroidogenic gene expression. Phosphorylates JUN and RUNX2. Seems to negatively regulate apoptosis by promoting FADD phosphorylation. Enhances androgen receptor-mediated transcription. May act as a transcriptional corepressor for NK homeodomain transcription factors. The phosphorylation of NR5A1 activates SF1 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation. In osteoblasts, supports transcription activation: phosphorylates RUNX2 that synergizes with SPEN/MINT to enhance FGFR2-mediated activation of the osteocalcin FGF-responsive element (OCFRE).

Molecular Function

ATP binding

protein kinase activity

protein serine/threonine kinase activity

Subcellular Location

Nucleus, cytoplasm

Expression

Overexpressed in multidrug resistant cells. Highly expressed in heart and skeletal muscle, and at lower levels in placenta, pancreas, brain, spleen, prostate, thymus, testis, small intestine, colon and leukocytes. Not found in liver and lung.

Structure

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. HIPK subfamily.

Alternative Nomenclature

Androgen receptor interacting nuclear protein kinase antibody

ANPK antibody

DYRK6 antibody

Fas-interacting serine/threonine-protein kinase antibody

FIST antibody

FIST3 antibody

Hipk3 antibody

homeodomain interacting protein kinase 3 antibody

Homolog of protein kinase YAK1 antibody

PKY antibody

RP24 297H17.5 antibody

YAK1 antibody