General Information

Product

GRK6 Antibody

Description

Affinity Purified G protein-coupled receptor kinase 6 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 500-550 on human G protein-coupled receptor kinase 6 protein.

Accession #

NCBI: NP_002073

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5-1.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Purification Method

Immobilized antigen affinity chromatography

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:50,000

Immunoprecipitation

1:200

Western Blot

1:2,500-1:5,000

Protein

Uniprot #

P43250

Overview

Specifically phosphorylates the activated forms of G protein-coupled receptors. Such receptor phosphorylation initiates beta-arrestin-mediated receptor desensitization, internalization, and signaling events leading to their desensitization. Seems to be involved in the desensitization of D2-like dopamine receptors in striatum and chemokine receptor CXCR4 which is critical for CXCL12-induced cell chemotaxis. Phosphorylates rhodopsin (RHO) (in vitro) and a non G-protein-coupled receptor: LRP6 during Wnt signaling (in vitro).

Molecular Function

ATP binding

beta-adrenergic receptor kinase activity

G-protein coupled receptor kinase activity

Subcellular Location

Membrane; Lipid-anchor

Expression

Widely expressed.

Structure

Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. GPRK subfamily.

Alternative Nomenclature

G protein coupled receptor kinase 6 antibody

G protein coupled receptor kinase GRK6 antibody

Gprk6 antibody

Grk6 antibody