General Information

Product

CHMP2B Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 200-250 on human Vacuolar-sorting Protein SNF8 protein.

Accession #

NCBI: NP_009172

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5-1.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Purification Method

Immobilized antigen affinity chromatography

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:50,000

Immunoprecipitation

1:200

Western Blot

1:2,500-1:5,000

Protein

Uniprot #

Q96H20

Overview

Component of the endosomal sorting complex required for transport II (ESCRT-II), which is required for multivesicular body (MVB) formation and sorting of endosomal cargo proteins into MVBs. The MVB pathway mediates delivery of transmembrane proteins into the lumen of the lysosome for degradation. The ESCRT-II complex is probably involved in the recruitment of the ESCRT-III complex. The ESCRT-II complex may also play a role in transcription regulation by participating in derepression of transcription by RNA polymerase II, possibly via its interaction with ELL. Required for degradation of both endocytosed EGF and EGFR, but not for the EGFR ligand-mediated internalization. It is also required for the degradation of CXCR4. Required for the exosomal release of SDCBP, CD63 and syndecan (PubMed:22660413).

Molecular Function

channel regulator activity

protein C-terminus binding

protein homodimerization activity

protein N-terminus binding

transcription factor binding

Subcellular Location

Endosome membrane

Late endosome membrane

Expression

Component of the endosomal sorting complex required for transport II (ESCRT-II), composed of SNF8, VPS25 and VPS36. SNF8 is essential for the stability of the ESCRT-II complex. ESCRT-II interacts with ELL. Interacts with TSG101 (via the C-terminal domain). Interacts with RILPL1 (via the N-terminal domain); which recruits ESCRT-II to the endosome membranes. Interacts with 14-3-3 proteins.

Structure

Belongs to the SNF8 family.

Alternative Nomenclature

ALS17 antibody

Charged multivesicular body protein 2b antibody

CHM2B_HUMAN antibody

CHMP family, member 2B antibody

CHMP2.5 antibody

CHMP2b antibody

Chromatin modifying protein 2b antibody

DMT1 antibody

hVps2-2 antibody

Vacuolar protein sorting 2, yeast, homolog of, B antibody

Vacuolar protein sorting 2-2 antibody

Vacuolar protein sorting-associated protein 2-2 antibody

VPS2 homolog B antibody

Vps2-2 antibody

VPS2B antibody