General Information

Product

AMPK alpha 1 Antibody

Description

Affinity Purified 5"-AMP-activated protein kinase catalytic subunit alpha-1 Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 500-550 on rat 5"-AMP-activated protein kinase catalytic subunit alpha-1 protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5-1.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Purification Method

Immobilized antigen affinity chromatography

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:50,000

Immunoprecipitation

1:200

Western Blot

1:2,500-1:5,000

Protein

Uniprot #

P54645

Overview

Catalytic subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation.

Molecular Function

[acetyl-CoA carboxylase] kinase activity

[hydroxymethylglutaryl-CoA reductase (NADPH)] kinase activity

AMP-activated protein kinase activity

ATP binding

chromatin binding

Subcellular Location

Nucleus, Cytoplasm

Expression

Low expression in kidney, liver, lung, heart and brain.

Structure

The AIS (autoinhibitory sequence) region shows some sequence similarity with the ubiquitin-associated domains and represses kinase activity.

Alternative Nomenclature

5 AMP activated protein kinase alpha 1catalytic subunit antibody

AAPK1 antibody

ACACA kinase antibody

acetyl CoA carboxylase kinase antibody

AMP -activate kinase alpha 1 subunit antibody

AMPK 1 antibody

AMPK alpha 1 antibody

HMG CoA reductase kinase antibody

HMGCR kinase antibody

hormone sensitive lipase kinase antibody

Hydroxymethylglutaryl CoA reductase kinase antibody

im:7154392 antibody

kinase AMPK alpha1 antibody

PRKAA 1 antibody

Protein kinase AMP activated alpha 1 catalytic subunit antibody

SNF1A antibody

Tau protein kinase PRKAA1 antibody