General Information

Product

HIPK2 Antibody

Description

Affinity Purified Homeodomain-interacting protein kinase 2 Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 1150-1198 on human Homeodomain-interacting protein kinase 2 protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5-1.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Purification Method

Immobilized antigen affinity chromatography

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:50,000

Immunoprecipitation

1:200

Western Blot

1:2,500-1:5,000

Protein

Uniprot #

Q9H2X6

Overview

Serine/threonine-protein kinase involved in transcription regulation, p53/TP53-mediated cellular apoptosis and regulation of the cell cycle. Acts as a corepressor of several transcription factors, including SMAD1 and POU4F1/Brn3a and probably NK homeodomain transcription factors. Phosphorylates PDX1, ATF1, PML, p53/TP53, CREB1, CTBP1, CBX4, RUNX1, EP300, CTNNB1, HMGA1 and ZBTB4. Inhibits cell growth and promotes apoptosis through the activation of p53/TP53 both at the transcription level and at the protein level (by phosphorylation and indirect acetylation). The phosphorylation of p53/TP53 may be mediated by a p53/TP53-HIPK2-AXIN1 complex. Involved in the response to hypoxia by acting as a transcriptional co-suppressor of HIF1A.

Molecular Function

ATP binding

protein kinase activity

protein serine/threonine kinase activity

RNA polymerase II activating transcription factor binding

RNA polymerase II transcription coactivator activity

SMAD binding

transcription corepressor activity

virion binding

Subcellular Location

Nucleus, PML body, Cytoplasm

Expression

Highly expressed in heart, muscle and kidney. Weakly expressed in a ubiquitous way. Down-regulated in several thyroid and breast tumors.

Alternative Nomenclature

HIPK 2 antibody

Homeodomain interacting protein kinase 2 antibody

Nbak1 antibody

Nuclear body-associated kinase 1 antibody

PRO0593 antibody

Sialophorin tail-associated nuclear serine/threonine-protein kinase antibody

Stank antibody