General Information

Product

Pin1 Antibody

Description

Peptidyl-prolyl cis-trans isomerase Pin1 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Monkey, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 50-100 or 118-168 on human peptidyl-prolyl cis-trans isomerase NIMA-interacting 1 protein.

Accession #

NCBI: XP_011526370.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.56-0.62 µg/µl in antibody stabilization buffer

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

UPI0005D038B7

Overview

Peptidyl-prolyl cis/trans isomerase (PPIase) that binds to and isomerizes specific phosphorylated Ser/Thr-Pro (pSer/Thr-Pro) motifs in a subset of proteins, resulting in conformational changes in the proteins (PubMed:21497122, PubMed:22033920). Displays a preference for an acidic residue N-terminal to the isomerized proline bond. Regulates mitosis presumably by interacting with NIMA and attenuating its mitosis-promoting activity. Down-regulates kinase activity of BTK (PubMed:16644721). Can transactivate multiple oncogenes and induce centrosome amplification, chromosome instability and cell transformation. Required for the efficient dephosphorylation and recycling of RAF1 after mitogen activation (PubMed:15664191). Binds and targets PML and BCL6 for degradation in a phosphorylation-dependent manner (PubMed:17828269). Acts as a regulator of JNK cascade by binding to phosphorylated FBXW7, disrupting FBXW7 dimerization and promoting FBXW7 autoubiquitination and degradation: degradation of FBXW7 leads to subsequent stabilization of JUN (PubMed:22608923).

Molecular Function

GTPase activating protein binding; mitogen-activated protein kinase kinase binding; peptidyl-prolyl cis-trans isomerase activity; phosphoserine binding; phosphothreonine binding

Subcellular Location

Nucleus; Nucleus speckle; Cytoplasm

Expression

The phosphorylated form at Ser-71 is expressed in normal breast tissue cells but not in breast cancer cells.

Structure

Interacts with STIL. Interacts with KIF20B. Interacts with NEK6. Interacts (via WW domain) with PRKX. Interacts with BTK. Interacts (via PpiC domain) with DAPK1. Interacts with the phosphorylated form of RAF1. Interacts (via WW domain) with ATCAY; upon NGF stimulation. Interacts with PML (isoform PML-4) and BCL-6. Interacts with FBXW7, disrupting FBXW7 dimerization and promoting FBXW7 autoubiquitination and degradation (PubMed:22608923).

Alternative Nomenclature

DOD antibody

DODO, Drosophila, homolog of antibody

FLJ40239 antibody

FLJ77628 antibody

MGC10717 antibody

NIMA interacting 1 antibody

Peptidyl prolyl cis trans isomerase NIMA interacting 1 antibody

Pin 1 antibody

PPIase Pin1 antibody

Prolyl isomerase antibody

Protein (peptidylprolyl cis/trans isomerase) NIMA interacting 1 antibody

Protein NIMA interacting 1 antibody

Rotamase Pin1 antibody

UBL5 antibody