General Information

Product

Deformed Epidermal Autoregulatory Factor 1 Antibody

Description

Affinity Purified Deformed epidermal autoregulatory factor 1 homolog Antibody

Verified Applications

ELISA, IHC,IP, WB

Host

Rabbit

Species Cross Reactivity

Mouse, Rat

Predicted:Human

Immunogen

Synthetic peptide taken within amino acid region 500-565 on mouse Deformed epidermal autoregulatory factor 1 homolog protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5-1.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Purification Method

Immobilized antigen affinity chromatography

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunocytochemistry

1:50-1:150

Immunohistochemistry

1:50-1:150

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

Q9Z1T5

Overview

Transcription factor that binds to sequence with multiple copies of 5"-TTC[CG]G-3" present in its own promoter and that of the HNRPA2B1 gene. Down-regulates transcription of these genes. Binds to the retinoic acid response element (RARE) 5"-AGGGTTCACCGAAAGTTCA-3". Activates the proenkephalin gene independently of promoter binding, probably through protein-protein interaction. When secreted, behaves as an inhibitor of cell proliferation, by arresting cells in the G0 or G1 phase. Required for neural tube closure and skeletal patterning. Regulates epithelial cell proliferation and side-branching in the mammary gland. Controls the expression of peripheral tissue antigens in pancreatic lymph nodes. Isoform 1 displays greater transcriptional activity than isoform 4. Isoform 4 may inhibit transcriptional activity of isoform 1 by interacting with isoform 1 and retaining it in the cytoplasm. Transcriptional activator of EIF4G3.

Molecular Function

DNA binding, metal ion binding, transcription factor activity, sequence-specific DNA binding

Subcellular Location

Cytoplasm, Nucleus, Secreted

Expression

Expressed in various tissues and cells such as in peripheral mononuclear cells and hormone-secreting pituitary cells. Expression in pancreatic lymph nodes of patients with type 1 diabetes is 20 times higher than in healthy controls. Highly expressed in fetal and adult brain.

Structure

Zinc-finger

Alternative Nomenclature

Deaf1 antibody

DEAF1 transcription factor antibody

Deformed epidermal autoregulatory factor 1 homolog antibody

Nuclear DEA1 related transcriptional regulator antibody

NUDR antibody

SPN antibody

Suppressin antibody

Zinc finger MYND domain containing protein 5 antibody

ZMYND5 antibody