General Information

Product

MeCP2 isoform 2 Antibody

Description

Affinity Purified Methyl-CpG-binding protein 2 isoform 2 Antibody

Verified Applications

ELISA, ICC/IF, IHC,IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 436-486 on Methyl-CpG-binding protein 2 isoform 2 protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.50µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

P51608

Overview

Chromosomal protein that binds to methylated DNA. It can bind specifically to a single methyl-CpG pair. It is not influenced by sequences flanking the methyl-CpGs. Mediates transcriptional repression through interaction with histone deacetylase and the corepressor SIN3A. Binds both 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC)-containing DNA, with a preference for 5-methylcytosine (5mC) .

Molecular Function

Chromatin binding, DNA binding, mRNA binding, double-stranded methylated DNA binding, poly(A) RNA binding, siRNA binding

Subcellular Location

Nucleus (Note: Colocalized with methyl-CpG in the genome)

Expression

Present in all adult somatic tissues tested.

Alternative Nomenclature

AUTSX 3 antibody

Mbd5 antibody

MeCP2 antibody

Methyl CpG binding protein 2 (Rett syndrome) antibody

MRX 16 antibody

MRX 79 antibody

MRXS 13 antibody

MRXSL antibody

PPMX antibody

RS antibody

RTS antibody

RTT antibody

WBP10 antibody