General Information

Product

USH2A Antibody

Description

Affinity Purified Usherin isoform A Antibody Mid-Region

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Rat

Immunogen

Synthetic peptide taken within amino acid region 255-305 on human Usherin isoform A protein.

Accession #

NCBI: NP_009054.5

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.62 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

Mid-Region

Storage

-20⁰C for long term storage

Application Protocol

Confocal Microscopy

1:250

DB

1:4,000

ELISA

1:4,000

Immunocytochemistry

1:250

Immunofluorescence

1:250

Immunohistochemistry

1:250

Immunoprecipitation

1:200

Western Blot

1:500. Detects apparent band of 186 kDa.

Protein

Uniprot #

O75445

Overview

Involved in hearing and vision.

Molecular Function

collagen binding, myosin binding

Subcellular Location

Cell projection › stereocilium membrane; Single-pass type I membrane protein

Expression

Present in the basement membrane of many, but not all tissues. Expressed in retina, cochlea, small and large intestine, pancreas, bladder, prostate, esophagus, trachea, thymus, salivary glands, placenta, ovary, fallopian tube, uterus and testis.

Structure

Interacts with collagen IV and fibronectin via its laminin EGF-like domains. Interaction with collagen may be required for stable integration into the basement membrane. Interacts with USH1C and WHRN. Interacts with NINL. Interacts with PDZD7.

Alternative Nomenclature

dJ1111A8.1 antibody

US1 antibody

USH1A antibody

Usher syndrome type Ia protein antibody

Usher syndrome type-1A protein antibody

Usherin antibody