General Information

Product

ULK3 Antibody

Description

Affinity Purified Serine/Threonine-protein kinase ULK3 Antibody C-epitope

Verified Applications

ELISA, IHC, IP,WB

Host

Rabbit

Species Cross Reactivity

Bovine, Human, Monkey, Mouse, Rat

Immunogen

Synthetic peptide corresponding to a region within C terminal amino acids 452-472 of Mouse ULK3, coupled to a carrier protein.

Accession #

NCBI: NP_001258064.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.65 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

C-epitope

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

D3ZHP7

Overview

Serine/threonine protein kinase that acts as a regulator of Sonic hedgehog (SHH) signaling and autophagy. Acts as a negative regulator of SHH signaling in the absence of SHH ligand: interacts with SUFU, thereby inactivating the protein kinase activity and preventing phosphorylation of GLI proteins (GLI1, GLI2 and/or GLI3). Positively regulates SHH signaling in the presence of SHH: dissociates from SUFU, autophosphorylates and mediates phosphorylation of GLI2, activating it and promoting its nuclear translocation. Phosphorylates in vitro GLI2, as well as GLI1 and GLI3, although less efficiently. Also acts as a regulator of autophagy: following cellular senescence, able to induce autophagy.

Molecular Function

ATP-binding; Nucleotide-binding; Serine/threonine-protein kinase

Subcellular Location

Cytoplasm

Expression

Widely expressed. Highest levels observed in fetal brain. In adult tissues, high levels in brain, liver and kidney, moderate levels in testis and adrenal gland and low levels in heart, lung, stomach, thymus, prostate and placenta. In the brain, highest expression in hippocampus, high levels also detected in the cerebellum, olfactory bulb and optic nerve. In the central nervous system, lowest levels in the spinal cord.

Structure

Interacts (via protein kinase domain) with SUFU.

Alternative Nomenclature

DKFZP434C131 antibody

FLJ90566 antibody

Serine/threonine-protein kinase ULK3 antibody

Ulk3 antibody

unc 51 like kinase 3 (C. elegans) antibody

Unc-51-like kinase 3 antibody