General Information

Product

MEK1 Antibody (MAP2K1)

Description

Affinity Purified Mitogen-activated protein kinase kinase 1 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Chicken

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 300-350 on human Mitogen-activated protein kinase kinase 1.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgY

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

Q02750

Overview

Dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. Binding of extracellular ligands such as growth factors, cytokines and hormones to their cell-surface receptors activates RAS and this initiates RAF1 activation.

Molecular Function

Kinase, Serine/threonine-protein kinase, Transferase, Tyrosine-protein kinase

Subcellular Location

Cytoplasm, Cytoskeleton, Membrane, Nucleus

Expression

Widely expressed, with extremely low levels in brain.

Structure

The proline-rich region localized between residues 270 and 307 is important for binding to RAF1 and activation of MAP2K1/MEK1.

Alternative Nomenclature

Dual specificity mitogen activated protein kinase kinase 1 antibodyERK activator kinase 1 antibodyMAP kinase/Erk kinase 1 antibodyMAP2K1 antibodyMAPKK 1 antibodyMAPKK1 antibodyMEKK1 antibodyMitogen activated protein kinase kinase 1 antibody MKK1 antibodyPRKMK1 antibody

Protein kinase mitogen activated, kinase 1 antibody