General Information

Product

KIRREL Antibody

Description

Affinity Purified Kin of IRRE-like protein 1 Antibody

Verified Applications

CM, ELISA, ICC, IF, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Recombinant protein

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.45-0.65 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

Confocal Microscopy

1:250

DOT Blot

1:10,000

ELISA

1:10,000

Immunocytochemistry

1:250

Immunofluorescence

1:250

Immunohistochemistry

1:250

Immunoprecipitation

1:250

Western Blot

1:250

Protein

Uniprot #

Q80W68

Overview

Plays a significant role in the normal development and function of the glomerular permeability. Is a signaling protein that needs the presence of TEC kinases to fully trans-activate the transcription factor AP-1.

Molecular Function

Myosin Binding

Subcellular Location

Cell membrane; Single-pass type I membrane protein

Expression

Abundantly expressed in kidney. Specifically expressed in podocytes of kidney glomeruli.

Structure

Interacts with TJP1/ZO-1 and with NPHS2/podocin (via the C-terminus). Interacts with NPHS1/nephrin (via the Ig-like domains); this interaction is dependent on KIRREL glycosylation. Homodimer (via the Ig-like domains). Interacts when tyrosine-phosphorylated with GRB2.

Alternative Nomenclature

FLJ10845 antibody

Kin of IRRE like (Drosophila) antibody

Kin of IRRE like antibody

Kin of irregular chiasm-like protein 1 antibody

Kirrel antibody

MGC129542 antibody

MGC129543 antibody

NEPH 1 antibody

Nephrin like protein 1 antibody

Nephrin related antibody