General Information

Product

MAPK7 Antibody

Description

Affinity Purified Mitogen-activated protein kinase 7 Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 700-750 on human Mitogen-activated protein kinase 7.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

Q13164

Overview

Plays a role in various cellular processes such as proliferation, differentiation and cell survival. The upstream activator of MAPK7 is the MAPK kinase MAP2K5. Upon activation, it translocates to the nucleus and phosphorylates various downstream targets including MEF2C. EGF activates MAPK7 through a Ras-independent and MAP2K5-dependent pathway. May have a role in muscle cell differentiation. May be important for endothelial function and maintenance of blood vessel integrity. MAP2K5 and MAPK7 interact specifically with one another and not with MEK1/ERK1 or MEK2/ERK2 pathways. Phosphorylates SGK1 at Ser-78 and this is required for growth factor-induced cell cycle progression. Involved in the regulation of p53/TP53 by disrupting the PML-MDM2 interaction.

Molecular Function

Kinase, Serine/threonine-protein kinase, Transferase

Subcellular Location

Cytoplasm, Nucleus, PML body

Expression

Expressed in many adult tissues. Abundant in heart, placenta, lung, kidney and skeletal muscle. Not detectable in liver.

Structure

The second proline-rich region may interact with actin targeting the kinase to a specific location in the cell.

The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

Alternative Nomenclature

Big MAP kinase 1 antibodyBMK 1 kinase antibodyERK 5 antibodyExtracellular signal regulated kinase 5 antibodyMAP kinase 7 antibodyMitogen-activated protein kinase 7 antibodyPRKM 7 antibody PROTEIN KINASE, MITOGEN-ACTIVATED, 7 antibody