General Information

Product

IRE1A Antibody

Description

Affinity Purified Serine/Threonine-protein kinase/endoribonuclease IRE1 Antibody

Verified Applications

ELISA, IP, WB

Host

Chicken

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide corresponding to unique amino acid sequence within amino acid region 900-977on human IRE1A protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.59-0.68 µg/µl in antibody stabilization buffer

Immunoglobulin

IgY

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

O75460

Overview

Senses unfolded proteins in the lumen of the endoplasmic reticulum via its N-terminal domain which leads to enzyme auto-activation. The active endoribonuclease domain splices XBP1 mRNA to generate a new C-terminus, converting it into a potent unfolded-protein response transcriptional activator and triggering growth arrest and apoptosis.

Molecular Function

Hydrolase, Kinase, Serine/threonine-protein kinase, Transferase

Subcellular Location

Endoplasmic reticulum membrane; Single-pass type 1 membrane protein

Expression

Ubiquitously expressed. High levels observed in pancreatic tissue.

Structure

Interacts with DAB2IP (via PH domain); the interaction occurs in a ER stress-induced dependent manner and is required for subsequent recruitment of TRAF2 to ERN1. Homodimer; disulfide-linked. Dimer formation is driven by hydrophobic interactions within the N-terminal luminal domains and stabilized by disulfide bridges. Also binds, HSPA5, a negative regulator of the unfolded protein response.

Alternative Nomenclature

Endoplasmic reticulum (ER) to nucleus signalling 1 antibody

Endoribonuclease antibodyER to nucleus signaling 1 antibodyERN 1 antibody hIRE 1p antibody Inositol requiring 1 antibody Inositol requiring protein 1 antibodyinositol-requiring enzyme 1 antibody IRE 1 antibodyIRE 1a antibodyIRE 1P antibodyIre1 alpha antibody

MGC163277 antibodyMGC163279 antibodyProtein kinase/endoribonuclease antibodyRGD1559716 antibodySerine/threonine protein kinase/endoribonuclease IRE1 antibody