General Information

Product

Eprhin Receptor B3 Antibody Affinity Purified

Description

Affinity Purified Ephrin type-B receptor 3 precursor Antibody

Verified Applications

ELISA, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 100-200 on EphBR3 protein.

Accession #

NCBI: NP_034273

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.58-0.61 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:750

Protein

Uniprot #

P54754

Overview

Receptor tyrosine kinase which binds promiscuously transmembrane ephrin-B family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Generally has an overlapping and redundant function with EPHB2. Like EPHB2, functions in axon guidance during development regulating for instance the neurons forming the corpus callosum and the anterior commissure, 2 major interhemispheric connections between the temporal lobes of the cerebral cortex. In addition to its role in axon guidance plays also an important redundant role with other ephrin-B receptors in development and maturation of dendritic spines and the formation of excitatory synapses. Controls other aspects of development through regulation of cell migration and positioning. This includes angiogenesis, palate development and thymic epithelium development for instance. Forward and reverse signaling through the EFNB2/EPHB3 complex also regulate migration and adhesion of cells that tubularize the urethra and septate the cloaca. Finally, plays an important role in intestinal epithelium differentiation segregating progenitor from differentiated cells in the crypt.

Molecular Function

ATP-binding, Nucleotide-binding

Subcellular Location

Cell membrane; Single-pass type I membrane protein; Early endosome membrane; Dendrite

Expression

Expressed in cells of the retinal ganglion cell layer during retinal axon guidance to the optic disk. Expressed by Paneth and progenitor cells in the crypts of the intestinal epithelium (at protein level).

Structure

Contains 1 Eph LBD domain; Contains 2 fibronectin type-III domains; Contains 1 protein kinase domain; Contains 1 SAM domain

Alternative Nomenclature

Cek10 antibody

EK2 antibody

Embryonic kinase 2 antibody

EPH Like Tyrosine Kinase 2 antibody

EPH receptor B3 antibody

EPH-like kinase 2 antibody

ephb3 antibody

Ephrin type-B receptor 3 antibody

ETK2 antibody

hEK2 antibody

Human Embryo Kinase 2 antibody

Mdk5 antibody

Sek4 antibody

TYRO6 antibody

Tyrosine protein kinase receptor HEK2 antibody

Tyrosine-protein kinase TYRO6 antibody