General Information

Product

Eprhin Receptor B4 Antibody

Description

Affinity Purified Ephrin type-B receptor 4 precursor Antibody

Verified Applications

ELISA, IP,WB

Host

Rabbit

Species Cross Reactivity

Human

Immunogen

Synthetic peptide taken within amino acid region 900-984 on human Ephrin B4 receptor protein.

Accession #

NCBI: NP_004435

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.62 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

P54760

Overview

Receptor tyrosine kinase which binds promiscuously transmembrane ephrin-B family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Together with its cognate ligand/functional ligand EFNB2 plays a central role in heart morphogenesis and angiogenesis through regulation of cell adhesion and cell migration. EPHB4-mediated forward signaling controls cellular repulsion and segregation form EFNB2-expressing cells. Plays also a role in postnatal blood vessel remodeling, morphogenesis and permeability and is thus important in the context of tumor angiogenesis.

Molecular Function

ATP-binding, Nucleotide-binding

Subcellular Location

Cell Membrane

Expression

Abundantly expressed in placenta but also detected in kidney, liver, lung, pancreas, skeletal muscle and heart. Expressed in primitive and myeloid, but not lymphoid, hematopoietic cells. Also observed in cell lines derived from liver, breast, colon, lung, melanocyte and cervix.

Structure

Heterotetramer upon binding of the ligand. The heterotetramer is composed of an ephrin dimer and a receptor dimer. Oligomerization is probably required to induce biological responses.

Alternative Nomenclature

Ephb4 antibody

Ephrin type-B receptor 4 antibody

Hepatoma Transmembrane Kinase antibody

HTK antibody

MYK 1 antibody

MYK1 antibody

TYRO 11 antibody

Tyrosine-protein kinase receptor HTK antibody

Tyrosine-protein kinase TYRO11 antibody