General Information

Product

UBR1 Antibody

Description

Affinity Purified E3 ubiquitin-protein ligase UBR1 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 1260-1500 on human UBR1 protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.68-0.70 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:4,000

ELISA

1:4,000

Immunohistochemistry

1:50-:150

Western Blot

1:500

Protein

Uniprot #

Q8IWV7

Overview

E3 ubiquitin-protein ligase which is a component of the N-end rule pathway. Recognizes and binds to proteins bearing specific N-terminal residues that are destabilizing according to the N-end rule, leading to their ubiquitination and subsequent degradation. May be involved in pancreatic homeostasis. Binds leucine and is a negative regulator of the leucine-mTOR signaling pathway, thereby controlling cell growth.

Molecular Function

Leucine binding; Ubiquitin-protein transferase activity; ligase activity; Zinc ion binding

Subcellular Location

Cytosol

Expression

Broadly expressed, with highest levels in skeletal muscle, kidney and pancreas. Present in acinar cells of the pancreas (at protein level).

Structure

The RING-H2 zinc finger is an atypical RING finger with a His ligand in place of the fourth Cys of the classical motif.

The UBR-type zinc finger forms a pocket that mediates recognition of type 1 N-degrons. It exhibits preference for Arginine in first position, has poor affinity for histidine, and doesn"t bind acetylated peptides.

Alternative Nomenclature

E3 ubiquitin-protein ligase UBR1 antibody

JBS antibody

N-recognin-1 antibody

ubiquitin protein ligase E3 component n-recognin 1 antibody

Ubiquitin-protein ligase E3-alpha-1 antibody UBR1 antibody