General Information

Product

PERK Antibody

Description

Affinity Purified Eukaryotic translation initiation factor 2-alpha kinase 3 Antibody

Verified Applications

DB, ELISA, IHC, IP,WB

Host

Chicken

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 1000-1050 on human Eukaryotic translation initiation factor 2-alpha kinase 3 protein.

Accession #

NCBI: NP_004827

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.68-0.70 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:4,000

ELISA

1:4,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

Q9NZJ5

Overview

Phosphorylates the alpha subunit of eukaryotic translation-initiation factor 2 (EIF2), leading to its inactivation and thus to a rapid reduction of translational initiation and repression of global protein synthesis. Serves as a critical effector of unfolded protein response (UPR)-induced G1 growth arrest due to the loss of cyclin-D1 (CCND1). Involved in control of mitochondrial morphology and function.

Molecular Function

Kinase, Serine/threonine-protein kinase, Transferase

Subcellular Location

Endoplasmic reticulum membrane; Single-pass type I membrane protein

Expression

Ubiquitous. A high level expression is seen in secretory tissues.

Structure

Forms dimers with HSPA5/BIP in resting cells. Oligomerizes in ER-stressed cells. Interacts with DNAJC3 and MFN2.

Alternative Nomenclature

DKFZp781H1925 antibody

E2AK3_HUMAN antibody

EC 2.7.11.1 antibody

Eif2ak3 antibody

Eukaryotic translation initiation factor 2 alpha kinase 3 antibody

Heme regulated EIF2 alpha kinase antibody

HRI antibody

HsPEK antibody

Pancreatic eIF2 alpha kinase antibody

PEK antibody

PRKR like endoplasmic reticulum kinase antibody

WRS antibody