General Information

Product

ENT1 Antibody

Description

Affinity Purified Equilibrative nitrobenzylthioinosine-sensitive nucleoside transporter ENT1 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid reigon 410-460 on mouse Equilibrative nitrobenzylthioinosine-sensitive nucleoside transporter ENT1 protein.

Accession #

GenBank: AAF64035.2

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

Q9JIM1

Overview

Mediates both influx and efflux of nucleosides across the membrane (equilibrative transporter). It is sensitive (ES) to low concentrations of the inhibitor nitrobenzylmercaptopurine riboside (NBMPR) and is sodium-independent. It has a higher affinity for adenosine. Resistant to dipyridamole and dilazep inhibition (anticancer chemotherapeutics drugs).

Molecular Function

Nucleoside transmembrane transporter activity

Subcellular Location

Cell membrane; Basolateral cell membrane; Apical cell membrane

Expression

Highly expressed in heart, spleen, lung, liver and testis. Lower level of expression in brain and kidney.

Structure

Belongs to the SLC29A/ENT transporter (TC 2.A.57) family.

Alternative Nomenclature

Equilibrative NBMPR-sensitive nucleoside transporter antibody

Equilibrative nitrobenzylmercaptopurine riboside-sensitive nucleoside transporter antibody

Equilibrative nucleoside transporter 1 antibody

es-type antibody

Nucleoside transporter, es-type antibody

Slc29a1 antibody

solute carrier family 29 (equilibrative nucleoside transporter), member 1 antibody

Solute carrier family 29 member 1 antibody