General Information

Product

PDE4A4 Antibody

Description

Affinity Purified cAMP specific phosphodiesterase 4A variant pde46 Antibody

Verified Applications

CM, ELISA, ICC/IF, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rabbit

Immunogen

N-terminus PDE4A4 synthetic cyclic peptide

Accession #

GenBank: EAW74861.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

1.00µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

Confocal Microscopy

1:100

DB

1:10,000

ELISA

1:10,000

Immunocytochemistry

1:100

Immunofluorescence

1:100

Immunohistochemistry

1:100

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

P27815

Overview

Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes.

Molecular Function

Hydrolase, 3",5"-cyclic-AMP phosphodiesterase activity, cAMP binding

Subcellular Location

Isoform 1: Cytoplasm › perinuclear region, Isoform 2: Cytoplasm, Cell projection, Isoform 4: Membrane; Peripheral membrane protein Isoform 6 : Cytoplasm › perinuclear region Isoform 7 : Cytoplasm. Membrane

Expression

Isoform 1 is widely expressed. Isoform 2 is abundant in liver, stomach, testis, thyroid and adrenal glands. It is also found in placenta, kidney, pancreas, ovary, uterus, skin, monocytes, mast cells, macrophages, as well as in bronchial smooth muscle. Isoform 6 is expressed at high levels in the heart and small intestine. It is also found in the brain, kidney, spleen, colon, salivary gland, ovary and peripheral blood lymphocytes. Isoform 7 is expressed predominantly in skeletal muscle and brain and at lower levels in the testis. Isoform 7 is expressed in the brain. Found in specific neuronal subpopulations in cortex, spinal cord and cerebellum (at protein level).

Structure

Isoform 1 interacts with LYN. Isoform 2 and isoform 6 interact weakly with LYN. Isoform 1, isoform 2 and isoform 6 interact with ARRB2.

Alternative Nomenclature

DPDE2 antibody

PDE4A antibody

Phosphodiesterase 4 antibody

Phosphodiesterase 4A antibody

Phosphodiesterse Type 4A4 antibody