General Information

Product

Phospho-TICAM2 Antibody

Description

Affinity Purified Phosphorylated TRIF-related adapter molecule Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Phosphorylated synthetic peptide corresponding to unique amino acid sequences on TRIF-related adapter molecule protein.

Accession #

GenBank: AAO74498.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.50-0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500-1:750

Protein

Uniprot #

Q86XR7

Overview

Functions as sorting adapter in LPS-TLR4 signaling to regulate the MYD88-independent pathway during the innate immune response to LPS. Physically bridges TLR4 and TICAM1 and functionally transmits LPS-TRL4 signal to TICAM1; signaling is proposed to occur in early endosomes after endocytosis of TLR4.

Molecular Function

phospholipid binding, signal transducer activity

Subcellular Location

Isoform 1 : Cytoplasm. Golgi apparatus. Cell membrane. Endoplasmic reticulum. Early endosome membrane. Late endosome membrane, Isoform 2 : Endoplasmic reticulum. Early endosome membrane. Late endosome membrane.

Expression

Expressed in spleen, prostate, testis, uterus, small intestine, colon, peripheral blood leukocytes, heart, placenta, lung, liver, skeletal muscle, and pancreas Isoform 2 is ubiquitously expressed (at lower levels than isoform 1).

Structure

Homodimer. Interacts with TLR4, TICAM1, IRF3 and IRF7 in response to LPS. Interacts with IL1R1, IL1RAP, IRAK2, IRAK3 and TRAF6. Interacts with protein kinase-inactive mutants of IRAK1 and IRAK4. Isoform 1 interacts with isoform 2; the interaction occurs in late endosomes and dirupts the interaction between isoform 1 and TICAM1. Interacts with MYD88; the interaction decreases after IL-18 stimulation in time-dependent manner. Interacts with IL18R1 and IL8RAP.

Alternative Nomenclature

Putative NF-kappa-B-activating protein 502 antibody

TIR containing adapter molecule 2 antibody

TIR domain containing adaptor protein antibody

TIRAP3 antibody

TIRP antibody

Toll-like receptor adaptor protein 3 antibody

Toll/interleukin-1 receptor domain-containing protein antibody

TRAM antibody

TRIF-related adapter molecule antibody