General Information

Product

PDE4D5 Antibody

Description

Affinity Purified cAMP-specific phosphodiesterase PDE4D5 Antibody

Verified Applications

CM, ELISA, ICC, IF, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rabbit, Rat

Immunogen

A 20 amino acid synthetic peptide taken from the N-terminus corresopnding to the PDE4D5 variant.

Accession #

GenBank: AAC00069.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.65 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

Confocal Microscopy

1:200

DB

1:10,000

ELISA

1:10,000

Immunocytochemistry

1:200

Immunofluorescence

1:200

Immunohistochemistry

1:200

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

Q08499

Overview

Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes.

Molecular Function

Hydrolase, 3",5"-cyclic-AMP phosphodiesterase activity, cAMP binding

Subcellular Location

Apical cell membrane, Cytoplasm, Membrane

Expression

Expressed in colonic epithelial cells (at protein level). Widespread; most abundant in skeletal muscle. Isoform 6 is detected in brain. Isoform 8 is detected in brain, placenta, lung and kidney. Isoform 7 is detected in heart and skeletal muscle.

Structure

Homodimer for the long isoforms. Isoforms with truncated N-termini are monomeric. Isoform 3 is part of a ternary complex containing PRKAR2A, PRKAR2B and AKAP9. Interacts with PDE4DIP. Identified in a complex composed of RYR1, PDE4D, PKA, FKBP1A and protein phosphotase 1 (PP1) (By similarity). Isoform 5, isoform N3 and isoform 12 bind GNB2L1 via their unique N-terminus. Binds ARRB2. Interacts (via N-terminal region) with SHANK2 (via proline-rich region); the interaction is increased in a PKA-dependent manner.

Alternative Nomenclature

cAMP-specific 3"5"-cyclic phosphodiesterase 4D antibody

DPDE3 antibody

dunce-like phosphodiesterase E3 antibody

hPDE4D5 antibody

PDE4D antibody

PDE4DN2 antibody

Phosphodiesterase 4D antibody

Phosphodiesterase 4D cAMP-specific (dunce (Drosophila)-homolog phosphodiesterase E3) antibody

Phosphodiesterase 4D cAMP-specific antibody

STRK1 antibody