General Information

Product

SHANK3 Antibody

Description

Affinity Purified SH3 and multiple ankyrin repeat domains protein 3 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 1600-1650 on mouse SH3 and multiple ankyrin repeat domains protein 3.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Purification Method

Immobilized antigen affinity chromatography

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

Q4ACU6

Overview

Major scaffold postsynaptic density protein which interacts with multiple proteins and complexes to orchestrate the dendritic spine and synapse formation, maturation and maintenance. Interconnects receptors of the postsynaptic membrane including NMDA-type and metabotropic glutamate receptors via complexes with GKAP/PSD-95 and HOMER, respectively, and the actin-based cytoskeleton. Plays a role in the structural and functional organization of the dendritic spine and synaptic junction through the interaction with Arp2/3 and WAVE1 complex as well as the promotion of the F-actin clusters. By way of this control of actin dynamics, participates in the regulation of developing neurons growth cone motility and the NMDA receptor-signaling. Also modulates GRIA1 exocytosis and GRM5/MGLUR5 expression and signaling to control the AMPA and metabotropic glutamate receptor-mediated synaptic transmission and plasticity. May be required at an early stage of synapse formation and be inhibited by IGF1 to promote synapse maturation.

Molecular Function

GFAP/Homer scaffold activity, identical protein binding, ionotropic glutamate receptor binding, protein C-terminus binding, scaffold protein binding, SH3 domain binding, zinc ion binding

Subcellular Location

Cell junction, Cell membrane, Cell projection, Cytoplasm, Membrane, Postsynaptic cell membrane, Synapse

Expression

In brain, highly expressed in striatum, thalamus, hippocampus and granule cells of the cerebellum.

Structure

Belongs to the SHANK family.

Contains 6 ANK repeats.

Contains 1 PDZ (DHR) domain.

Contains 1 SAM (sterile alpha motif) domain.

Contains 1 SH3 domain.

Alternative Nomenclature

AI841104 antibody

DEL22q13.3 antibody

KIAA1650 antibody

Proline-rich synapse-associated protein 2 antibody

PSAP2 antibody

SH3 and multiple ankyrin repeat domains 3 antibody

SH3/ankyrin domain gene 3 antibody

SHAN3_HUMAN antibody

Shank postsynaptic density protein antibody

Shank3 antibody

Shank3b antibody

SPANK 2 antibody