General Information

Product

Phospho-Doublecortin Antibody

Description

Affinity Purified Phosphorylated Neuronal migration protein doublecortin Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 200-250 on human Mesencephalic astrocyte-derived neurotrophic factor.

Accession #

NCBI: NP_835365

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Modification

Phosphorylated

Purification Method

Immobilized antigen affinity chromatography

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:50,000

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

O43602

Overview

Microtubule-associated protein required for initial steps of neuronal dispersion and cortex lamination during cerebral cortex development. May act by competing with the putative neuronal protein kinase DCLK1 in binding to a target protein. May in that way participate in a signaling pathway that is crucial for neuronal interaction before and during migration, possibly as part of a calcium ion-dependent signal transduction pathway. May be part with PAFAH1B1/LIS-1 of overlapping, but distinct, signaling pathways that promote neuronal migration.

Molecular Function

Developmental protein

Subcellular Location

Cytoplasm, Cell projection

Expression

Highly expressed in neuronal cells of fetal brain (in the majority of cells of the cortical plate, intermediate zone and ventricular zone), but not expressed in other fetal tissues. In the adult, highly expressed in the brain frontal lobe, but very low expression in other regions of brain, and not detected in heart, placenta, lung, liver, skeletal muscles, kidney and pancreas.

Structure

Contains 2 doublecortin domains.

Alternative Nomenclature

DBCN antibodyDbct antibodyDC antibodyDCX antibody Doublecortex antibodyDoublin antibody Lis X antibody Lissencephalin X antibodyLissencephaly X linked doublecortin antibodyLISX antibodyNeuronal migration protein doublecortin antibodySCLH antibodyXLIS antibody