General Information

Product

Shadow Antibody

Description

Affinity Purified Shadow Antibody Mid Region

Verified Applications

CM, ELISA, ICC, IF, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Drosophila melanogaster (Fruit fly)

Immunogen

Synthetic peptide corresponding to unique amino acid sequence near mid region of Shadow protein.

Accession #

NCBI: NP_650123

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.7 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

N-epitope

Storage

-20⁰C for long term storage

Application Protocol

Confocal Microscopy

1:200

DB

1:10,000

ELISA

1:10,000

Immunocytochemistry

1:200

Immunohistochemistry

1:200

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

Q9VGH1

Overview

Required for CNS development: midline glial cells. Involved in the metabolism of insect hormones: responsible for ecdysteroid C2-hydroxylase activity. May be involved in the breakdown of synthetic insecticides.

Molecular Function

Monooxygenase, Oxidoreductase, ecdysteroid 2-hydroxylase activity

Subcellular Location

Mitochondrion membrane

Expression

Complex coexpression pattern of dib (disembodied) and sad (shade) in the early embryo that restricts to the prothoracic gland cells of the developing ring gland during late embryogenesis. In larvae and adult, coexpression is seen in prothoracic gland and follicle cells of the ovary. In adults, coexpression is seen in the follicle cells, sad only is expressed in nurse cells.

Alternative Nomenclature

CG14728 antibody

Cyp315a1 antibody

CYPCCCXVA1 antibody

Cytochrome P450 315a1 mitochondrial antibody

Karmoisin ghost antibody

LP13573p antibody

sad antibody

Sad PA antibody

shade antibody