FabGennix/TKV-101AP-免疫在线蚂蚁淘旗下平台

商品详细FabGennix/TKV-101AP

FabGennix/TKV-101AP

商品编号： TKV-101AP

品牌： FabGennix Inc

市场价： ¥5900.00

美元价： 2655.00

产地：美国(厂家直采)

公司：

产品分类：功能性抗体

公司分类： Functional_antibody

联系Q Q： 3392242852

电话号码： 4000-520-616

电子邮箱： info@ebiomall.com

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商品介绍

Description
QA_1
Q&A

General Information
Product	ThickveinsAntibody
Description	Affinity Purified Thickveins Antibody
Verified Applications	ELISA, IP, WB
Host	Rabbit
Species Cross Reactivity	Fruit Fly (Drosophilamelanogaster)
Immunogen	Synthetic peptide corresponding to unique amino acid sequences on C-terminus of pan Thickvein protein. Immunogenic peptide was covalently modified to achieve the desired antigenicity. Sequence: CVK GFR PPI PSR WQE DD
Accession #	NCBI: NP_787992.1
Physical Properties
Quantity	100 µg
Volume	200 µl
Concentration	0.70 µg/µl in antibody stabilization buffer
Form	Affinity Purified
Clonality	Polyclonal
Storage	-20⁰C for long term storage
Application Protocol
DB	1:10,000
ELISA	1:10,000
Immunoprecipitation	1:200
Western Blot	1:500 predictedmolecular weights: 57-64 kDa
Protein
Uniprot #	Q95SI0
Overview	ATP binding, protein kinase activity, receptor signaling protein serine/threonine kinase activity
Alternative Nomenclature	Thick veins protein antibody tkv antibody

Protein information supplied by UniProt

Relevance

Fruit fly (Drosophila melanogaster) ovaries contains two set of germline stem cells surrounded by a group of highly differentiated somatic cells that express genes for two phenotypes (hedgehog & wingless). The TGF beta super family member, decpentaplegic (dpp) or its homologue BMP2/4 is specifically required for maintenance and promote its cell division in the female germline (1, 2).The Signaling by TGF beta-related factors requires ligand-induced association between type I and type II transmembrane receptors that have endogenous serine/threonine kinases activity. In Drosophila, thickveins (tkv) and saxophone (sax) genes encode type I receptors that mediate signaling by decapentaplegic (dpp), a member of the bone morphogenetic protein (BMP) subgroup of TGF beta-type factors. Patterning the dorsal surface of fruitfly blastoderm embryo requires Decapentaplegic (Dpp) and Screw (Scw), two BMP family members. The signaling by these ligands is mediated by Bone morphogenentic protein (BMP) binding proteins Sog and Tsg. It is demonstrated that Tsg and Sog play essential role in transport of heterodimer Dpp/Sog signaling through the two type I BMP receptors Tkv and SAX (3). Over expression or mutation in dpp suppress germline stem cell differentiation. Dpp actions are mediated by its receptor Saxophone. The Saxophone gene is expressed ubiquitously. The Saxophone gene also gives two products Brk43E and Berk25 and both gene products inter acts with TGF super family peptide ligands Dpp. Mutations that completely abolish Saxophone activity causes phenotype that are similar to partial or complete loss of activity of the dpp ligand. The saxophone products are also have serine threonine kinase activity responsible for phsosphorylation and activation of the ligands including pMAD, Screw (Scw), and short gastrulation protein (Sog).

The product page for anti-Thickveins antibody mentions the suggested parameters for Western Blot applications. I was wondering what protein source was used to determine those? We are interested in using this antibody for Western Blot using whole fly or fly head extracts. I wanted to know if it has been tested against
1. The blot will have a better signal to noise ratio if head extracts are used. This will increase the specific activity of Tkv in sample.

The product page for anti-Thickveins antibody mentions the suggested parameters for Western Blot applications. I was wondering what protein source was used to determine those? We are interested in using this antibody for Western Blot using whole fly or fly head extracts. I wanted to know if it has been tested against
1. The blot will have a better signal to noise ratio if head extracts are used. This will increase the specific activity of Tkv in sample.

品牌介绍

苏州蚂蚁淘Fabgennix授权代理业务。Fabgennix授权代理， Fabgennix北京代理， Fabgennix华东代理，Fabgennix华北代理，Fabgennix华中代理，Fabgennix华南代理。 Fabgennix 我们提供高质量的研究工具，专家技术支持，相关的准确数据以及快速的全球运输。我们不断在我们的库存中添加新产品，包括一级和二级抗体，试剂，试剂盒，多肽和阳性对照。我们所有的抗体都在内部开发和表征。我们为我们的Single Source Antibodies™感到自豪。部分产品的详细列表：Fabgennix授权代理货号产品价格 FGI-1989 StripOBuffer Western Blot Stripping Buffer $150.00 HRP-1060 Rabbit Anti-Chicken IgG HRP $150.00 FGI-1943 SolObuffer Cell Lysis Kit $175.00 FGI-1963 DiluOBuffer - Antibody Dilution Buffer $185.00 HIL2-E96.1 Human IL-2 ELISA Kit $495.00