General Information

Product

RUNX2 Antibody

Description

Affinity Purified Runt-related transcription factor 2 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Bovine, Human, Monkey, Panda

Immunogen

Synthetic peptide taken within amino acid region 10-50 on human RUNX2 protein.

Accession #

NCBI: XP_001100855.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.53 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

UPI0000D9AD12

Overview

Transcription factor involved in osteoblastic differentiation and skeletal morphogenesis. Essential for the maturation of osteoblasts and both intramembranous and endochondral ossification. CBF binds to the core site, 5"-PYGPYGGT-3", of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, osteocalcin, osteopontin, bone sialoprotein, alpha 1(I) collagen, LCK, IL-3 and GM-CSF promoters. In osteoblasts, supports transcription activation: synergizes with SPEN/MINT to enhance FGFR2-mediated activation of the osteocalcin FGF-responsive element (OCFRE). Inhibits KAT6B-dependent transcriptional activation.

Molecular Function

DNA-binding; Transcription Regulation

Subcellular Location

Nucleus

Structure

Heterodimer of an alpha and a beta subunit. The alpha subunit binds DNA as a monomer and through the Runt domain. DNA-binding is increased by heterodimerization. Interacts with XRCC6 (Ku70) and XRCC5 (Ku80). Interacts with HIVEP3. Interacts with IFI204. I

Alternative Nomenclature

Acute myeloid leukemia 3 protein antibody

Alpha subunit 1 antibody

AML3 antibody

CBF alpha 1 antibody

CBFA1 antibody

CCD1 antibody

Cleidocranial dysplasia 1 antibody

Core binding factor runt domain alpha subunit 1 antibody

Core binding factor subunit alpha 1 antibody

Oncogene AML 3 antibody

OSF 2 antibody

Osteoblast specific transcription factor 2 antibody

PEBP2 alpha A antibody

PEBP2A1 antibody

PEBP2A2 antibody

Polyomavirus enhancer-binding protein 2 alpha A subunit antibody

Runt domain antibody

Runt related transcription factor 2 antibody SL3 3 enhancer factor 1 alpha A subunit antibody