General Information

Product

MRAP2 Antibody

Description

Affinity Purified Melanocortin-2 receptor accessory protein Antibody C-epitope

Verified Applications

CM, ELISA, ICC, IF, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 70-120 on mouse Melanocortin-2 receptor accessory protein.

Accession #

NCBI: AY079153

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.60-0.61 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

C-epitope

Storage

-20⁰C for long term storage

Application Protocol

Dot blot

1:10,000

ELISA

1:10,000

Immunocytochemistry

1:200

Immunofluorescence

1:200

Immunohistochemistry

1:200

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

Q9D159

Overview

Modulator of melanocortin receptors (MC1R, MC2R, MC3R, MC4R and MC5R). Acts by increasing ligand-sensitivity of melanocortin receptors and enhancing generation of cAMP by the receptors. Required both for MC2R trafficking to the cell surface of adrenal cells and for signaling in response to corticotropin (ACTH). May be involved in the intracellular trafficking pathways in adipocyte cells.

Molecular Function

Brown fat cell differentiation; Positive regulation of cAMP biosynthetic process; Protein localization to cell surface

Subcellular Location

Single-pass membrane protein; Endoplasmic Reticulum membrane

Expression

Largely restricted to fat tissues. Predominantly expressed in mouse epididymal (white adipose tissue) and interscapular (brown adipose tissue) fat pads. Expression is weak or absent in lung, spleen, intestine, kidney, heart and skeletal muscle.

Structure

Homodimer and heterodimer. Forms antiparallel homodimers and heterodimers with MRAP2. Interacts with MC1R, MC2R, MC3R, MC4R and MC5R.

Alternative Nomenclature

bA51G5.2 antibody

BMIQ18 antibody

C6orf117 antibody

MC2R accessory protein 2 antibody

Melanocortin-2 receptor accessory protein 2 antibody