General Information

Product

U2AF59 Antibody

Description

Affinity Purified Splicing factor U2AF 59 kDa subunit Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Saccharomyces cerevisiae (yeast)

Immunogen

Synthetic peptide taken within amino acid region 480-517on yeast U2AF59 protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.75-1.13 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:200

Western Blot

1:500-1:750

Protein

Uniprot #

P36629

Overview

Necessary for the splicing of pre-mRNA. The SF1-U2AF59-U2AF23 complex has a role in the recognition of the branch site (5"-UACUAAC-3"), the pyrimidine tract and the 3"-splice site at the 3"-end of introns.

Molecular Function

mRNA binding, snoRNA binding, nucleotide binding

Subcellular Location

Nucleus

Structure

Forms a heterodimer with the U2AF small subunit. Can also form a homodimer. U2AF large subunit (U2AF59), U2AF small subunit (U2AF23) and SF1 (bpb1) interact to form a complex required for complex A formation. Interacts with wat1/pop3.

Alternative Nomenclature

S. pombe splicing factor U2 59 kDa subunit

Schzosacharomyces pombe splicing factor U2 auxiliary factor

U2 auxiliary factor 59 kDa subunit

U2 snRNP auxiliary factor large subunit

mis11

prp2