General Information

Product

MAVS Antibody

Description

Affinity Purified Mitochondrial Anti Viral-Signaling Protein Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human

Immunogen

Synthetic peptide taken within amino acid region 500-540 on on human mitochondrial antiviral-signaling protein isoform 1.

Accession #

NCBI: NP_065797

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

Q7Z434

Overview

Required for innate immune defense against viruses. Acts downstream of DDX58/RIG-I and IFIH1/MDA5, which detect intracellular dsRNA produced during viral replication, to coordinate pathways leading to the activation of NF-kappa-B, IRF3 and IRF7, and to the subsequent induction of antiviral cytokines such as IFN-beta and RANTES (CCL5). Peroxisomal and mitochondrial MAVS act sequentially to create an antiviral cellular state. Upon viral infection, peroxisomal MAVS induces the rapid interferon-independent expression of defense factors that provide short-term protection, whereas mitochondrial MAVS activates an interferon-dependent signaling pathway with delayed kinetics, which amplifies and stabilizes the antiviral response. May activate the same pathways following detection of extracellular dsRNA by TLR3. May protect cells from apoptosis.

Molecular Function

Antiviral defense, Host-virus interaction, Immunity, Innate immunity

Subcellular Location

Mitochondrion outer membrane, Mitochondrion, Peroxisome

Expression

Present in T-cells, monocytes, epithelial cells and hepatocytes (at protein level). Ubiquitously expressed, with highest levels in heart, skeletal muscle, liver, placenta and peripheral blood leukocytes.

Structure

Both CARD and transmembrane domains are essential for antiviral function. The CARD domain is responsible for interaction with DDX58/RIG-I and IFIH1/MDA5.

The transmembrane domain and residues 300-444 are essential for its interaction with DHX58/LGP2.

Alternative Nomenclature

CARD adapter inducing interferon beta antibody

IFN B promoter stimulator 1 antibody

Interferon beta promoter stimulator protein 1 antibody

Ips 1 antibody

MAVS antibody

Mitochondrial anti viral signaling protein antibody

Putative NF kappa B activating protein 031N antibody

Virus induced signaling adapter antibodyVISA antibody