General Information

Product

Phospho MAPK14 Antibody Affinity Purified

Description

Affinity Purified Phospho p38 Mitogen Activated Protein Kinase 14 Antibody

Verified Applications

ELISA, IMM, WB

Host

Rabbit

Species Cross Reactivity

Human, Monkey, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 250-300 on human MAPK14 protein.

Accession #

NCBI: NP_001306

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.65-0.69 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:20,000-1:50,000

ELISA

1:20,000-1:50,000

Immunoprecipitation

1:200

Western Blot

1:500-1:1,000

Protein

Uniprot #

Q16539

Overview

Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK14 is one of the four p38 MAPKs which play an important role in the cascades of cellular responses evoked by extracellular stimuli such as proinflammatory cytokines or physical stress leading to direct activation of transcription factors. Accordingly, p38 MAPKs phosphorylate a broad range of proteins and it has been estimated that they may have approximately 200 to 300 substrates each. Some of the targets are downstream kinases which are activated through phosphorylation and further phosphorylate additional targets. MAPK14 interacts also with casein kinase II, leading to its activation through autophosphorylation and further phosphorylation of TP53/p53. In the cytoplasm, the p38 MAPK pathway is an important regulator of protein turnover. The p38 MAPKs may also have kinase-independent roles, which are thought to be due to the binding to targets in the absence of phosphorylation.

Molecular Function

ATP binding; MAP kinase activity; MAP kinase kinase activity; NFAT protein binding; Protein serine/threonine kinase activity

Subcellular Location

Cytoplasm; Nucleus

Expression

Brain, heart, placenta, pancreas and skeletal muscle. Expressed to a lesser extent in lung, liver and kidney.

Structure

Binds to a kinase interaction motif within the protein tyrosine phosphatase, PTPRR (By similarity). This interaction retains MAPK14 in the cytoplasm and prevents nuclear accumulation (By similarity). Interacts with SPAG9 and GADD45A (By similarity). Interacts with CDC25B, CDC25C, DUSP1, DUSP10, DUSP16, NP60, SUPT20H and TAB1. Interacts with casein kinase II subunits CSNK2A1 and CSNK2B.

Alternative Nomenclature

CSAID-binding protein antibody

CSBP1 antibody

CSBP2 antibody

Cytokine suppressive anti-inflammatory drug-binding protein antibody

EXIP antibody

MAP kinase 14 antibody

MAP kinase MXI2 antibody

MAP kinase p38 alpha antibody

MAPK14 antibody

MAX-interacting protein 2 antibody

Mitogen-activated protein kinase 14 antibody

Mitogen-activated protein kinase p38 alpha antibody

Mxi2 antibody

p38 antibody

p38 MAP kinase antibody

p38 mitogen activated protein kinase antibody

p38alpha Exip antibody

PRKM14 antibody

PRKM15 antibody

RK antibody

SAPK2A antibody

Stress-activated protein kinase 2a antibody