General Information

Product

CD300 Antibody

Description

Affinity Purified CMRF35-like molecule 1 / CD300LF Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Monkey

Immunogen

Synthetic peptide taken within amino acid region 230-290 on human CMRF35-like molecule 1 / CD300LF.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5-1.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Purification Method

Immobilized antigen affinity chromatography

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunocytochemistry

1:50-1:150

Immunohistochemistry

1:50-1:150

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

Q8TDQ1

Overview

Acts as an inhibitory receptor for myeloid cells and mast cells (PubMed:15549731). Positively regulates the phagocytosis of apoptotic cells (efferocytosis) via phosphatidylserine (PS) recognition; recognizes and binds PS as a ligand which is expressed on the surface of apoptotic cells. Plays an important role in the maintenance of immune homeostasis, by promoting macrophage-mediated efferocytosis and by inhibiting dendritic cell-mediated efferocytosis. Negatively regulates Fc epsilon receptor-dependent mast cell activation and allergic responses via binding to ceramide and sphingomyelin which act as ligands (PubMed:24035150). May act as a coreceptor for interleukin 4 (IL-4). Associates with and regulates IL-4 receptor alpha-mediated responses by augmenting IL-4- and IL-13-induced signaling. Negatively regulates the Toll-like receptor (TLR) signaling mediated by MYD88 and TRIF through activation of PTPN6/SHP-1 and PTPN11/SHP-2 (PubMed:22043923). Inhibits osteoclast formation. Induces macrophage cell death upon engagement.

Molecular Function

ceramide binding

interleukin-4 receptor binding

phosphatidylserine binding

Subcellular Location

Cell membrane; Single-pass type I membrane protein

Expression

Highly expressed in spleen, peripheral blood leukocyte and monocyte, and lung. Weakly expressed in thymus, heart, brain, placenta, liver, skeletal muscle, kidney, pancreas, prostate, testis, ovary, small intestine or colon. Expressed selectively in monocytes and monocyte-related cells.

Alternative Nomenclature

CD300 antigen like family member antibody

CD300 antigen-like family member F antibody

CD300f antibody

CD300LF antibody

CLM antibody

CLM-1 antibody

CMRF35-like molecule 1 antibody

IGSF antibody

IgSF13 antibody

Immune receptor expressed on myeloid cells 1 antibody

immunoglobin superfamily member antibody

Immunoglobulin superfamily member 13 antibody

Inhibitory receptor IREM1 antibody

IREM antibody

IREM-1 antibody

Nepmucin antibody

NK inhibitory receptor antibody

NKIR antibody

TREM antibody

triggering receptors expressed on myeloid cells antibody

UNQ3105/PRO10111 antibody