General Information

Product

VPRBP Antibody Affinity Purified

Description

Affinity Purified VPR Antibody

Verified Applications

ELISA, IMM, WB

Host

Rabbit

Species Cross Reactivity

Human Immunodeficiency Virus 1

Immunogen

Synthetic peptide taken within amino acid region 1-27 on human VPR protein.

Accession #

GenBank: AGV39791.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.52 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

T2D0A6

Overview

During virus entry, involved in the transport of the viral pre-integration (PIC) complex to the nucleus (PubMed:12417576). This function is crucial for viral infection of non-dividing macrophages (PubMed:12417576). May act directly at the nuclear pore complex, by binding nucleoporins phenylalanine-glycine (FG)-repeat regions. During virus replication, may deplete host UNG protein, and incude G2-M cell cycle arrest(PubMed:7666531, PubMed:7474100). Acts by targeting specific host proteins for degradation by the 26S proteasome, through association with the cellular CUL4A-DDB1 E3 ligase complex by direct interaction with host VPRPB/DCAF-1. Cell cycle arrest reportedly occurs within hours of infection and is not blocked by antiviral agents, suggesting that it is initiated by the Vpr carried into the virion. Additionally, Vpr induces apoptosis in a cell cycle dependent manner suggesting that these two effects are mechanistically linked. Detected in the serum and cerebrospinal fluid of AIDS patient, Vpr may also induce cell death to bystander cells (PubMed:11000244).

Molecular Function

Viral Life Cycle

Subcellular Location

Virion; Host nucleus; Host Extracellular Space

Structure

Homooligomer, may form homodimer (PubMed:7798208). Interacts with p6-gag region of the Pr55 Gag precursor protein through a (Leu-X-X)4 motif near the C terminus of the P6gag protein (PubMed:7474102). Interacts with host UNG (PubMed:9151883). May interact with host RAD23A/HHR23A (PubMed:9371639). Interacts with host VPRBP/DCAF1, leading to hijack the CUL4A-RBX1-DDB1-DCAF1/VPRBP complex, mediate ubiquitination of host proteins such as TERT and ZGPAT and arrest the cell cycle in G2 phase (PubMed:17314515, PubMed:17630831, PubMed:17314515).

Alternative Nomenclature

DCAF1 antibody

DDB1 and CUL4 associated factor 1 antibody

HIV-1 Vpr-binding protein antibody

KIAA0800 antibody

protein VPRBP antibody

RIP antibody

Vpr (HIV-1) binding protein antibody

VPR BP antibody

Vpr interacting protein antibody

Vpr-binding protein antibody