General Information

Product

CARD15 Antibody

Description

Affinity Purified Nucleotide-binding oligomerization domain-containing protein 2

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 700-780 on mouse Nucleotide-binding oligomerization domain-containing protein 2.

Accession #

GenBank: AAH44774

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5-1.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Purification Method

Immobilized antigen affinity chromatography

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:50,000

Immunoprecipitation

1:200

Western Blot

1:2,500-1:5,000

Protein

Uniprot #

Q8K3Z0

Overview

Involved in gastrointestinal immunity. Upon stimulation by muramyl dipeptide (MDP), a fragment of bacterial peptidoglycan, binds the proximal adapter receptor-interacting RIPK2, which recruits ubiquitin ligases as XIAP, BIRC2, BIRC3 and the LUBAC complex, triggering activation of MAP kinases and activation of NF-kappa-B signaling. This in turn leads to the transcriptional activation of hundreds of genes involved in immune response (PubMed:22607974). Required for MDP-induced NLRP1-dependent CASP1 activation and IL1B release in macrophages (PubMed:18511561).

Molecular Function

actin binding

ATP binding

CARD domain binding

endodeoxyribonuclease activity, producing 5"-phosphomonoesters

enzyme binding

Hsp70 protein binding

Hsp90 protein binding

muramyl dipeptide binding

peptidoglycan binding

protein kinase binding

Subcellular Location

Cytoplasm

Membrane

Basolateral cell membrane

Structure

The ATG16L1-binding motif mediates interaction with ATG16L1.

Intramolecular interactions between the N-terminal moiety and the leucine-rich repeats (LRR) may be important for autoinhibition in the absence of activating signal. In the absence of LRRs, the protein becomes a constitutive activator of CASP1 cleavage and proIL1B processing.

Alternative Nomenclature

ACUG antibody

Arthrocutaneouveal granulomatosis antibody

BLAU antibody

CARD15 antibody

Caspase recruitment domain protein 15 antibody

CD antibody

CLR16.3 antibody

IBD1 antibody

Inflammatory bowel disease protein 1 antibody

LRR containing protein antibody

NLR family, CARD domain containing 2 antibody

NLRC2 antibody

NOD like receptor C2 antibody

NOD2 antibody

NOD2B antibody

Nucleotide binding oligomerization domain, leucine rich repeat and CARD domain containing 2 antibody

PSORAS1 antibody