General Information

Product

Synaptophysin Antibody Affinity Purified C-epitope

Verified Applications

CM, ELISA, ICC, IF, IHC, IMM, WB

Host

Rabbit

Species Cross Reactivity

Chicken, Human, Monkey, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 250-350 on human Synaptophysin protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.60-0.62 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

C-epitope

Storage

-20⁰C for long term storage

Application Protocol

Confocal Microscopy

1:100

DB

1:10,000

ELISA

1:10,000

Immunocytochemistry

1:100

Immunofluorescence

1:100

Immunohistochemistry

1:100

Immunoprecipitation

1:125

Western Blot

1:1,000

Protein

Uniprot #

P08247

Overview

Possibly involved in structural functions as organizing other membrane components or in targeting the vesicles to the plasma membrane. Involved in the regulation of short-term and long-term synaptic plasticity (By similarity).

Molecular Function

Cholesterol binding, protein self-association, transporter activity

Subcellular Location

Cytoplasmic vesicle › secretory vesicle › synaptic vesicle membrane; Multi-pass membrane protein. Cell junction ›synapse › synaptosome

Expression

Characteristic of a type of small (30-80 nm) neurosecretory vesicles, including presynaptic vesicles, but also vesicles of various neuroendocrine cells of both neuronal and epithelial phenotype.

Structure

Homohexamer or homotetramer. Interacts with SRCIN1.

Alternative Nomenclature

Major synaptic vesicle protein P38 antibody

MRXSYP antibody

Syn p38 antibody

Synaptophysin antibody

SYP antibody

SYPH antibody

SypI antibody