General Information

Product

Phospho-JAK2 Antibody

Description

Affinity Purified Phosphorylated Tyrosine-protein kinase JAK2 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 980-1030 on human JAK2 protein. Phosphorylated 1008 & 1009 tyrosine residues.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

O60674

Overview

Non-receptor tyrosine kinase involved in various processes such as cell growth, development, differentiation or histone modifications. Mediates essential signaling events in both innate and adaptive immunity. In the cytoplasm, plays a pivotal role in signal transduction via its association with type I receptors such as growth hormone (GHR), prolactin (PRLR), leptin (LEPR), erythropoietin (EPOR), thrombopoietin (THPO); or type II receptors including IFN-alpha, IFN-beta, IFN-gamma and multiple interleukins (PubMed:7615558). Following ligand-binding to cell surface receptors, phosphorylates specific tyrosine residues on the cytoplasmic tails of the receptor, creating docking sites for STATs proteins (PubMed:9618263). Subsequently, phosphorylates the STATs proteins once they are recruited to the receptor.

Molecular Function

Adaptive immunity, Immunity, Innate immunity

Subcellular Location

Endomembrane system; Peripheral membrane protein; Cytoplasm; Nucleus

Expression

Ubiquitously expressed throughout most tissues.

Structure

Possesses 2 protein kinase domains. The second one probably contains the catalytic domain, while the presence of slight differences suggest a different role for protein kinase 1.

Alternative Nomenclature

JAK 2

Janus Activating Kinase 2

Janus kinase 2 (a protein tyrosine kinase)

Janus kinase 2

JTK 10

THCYT3

Tyrosine protein kinase JAK2