General Information

Product

PRAS40 Antibody

Description

Affinity Purified Proline-rich AKT1 substrate 1 isoform a Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 160-210 on human Proline-rich AKT1 substrate 1 isoform a protein.

Accession #

NCBI: NP_115751

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Purification Method

Immobilized antigen affinity chromatography

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

Q96B36

Overview

Subunit of mTORC1, which regulates cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino acids. Growth factor-stimulated mTORC1 activation involves a AKT1-mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Amino acid-signaling to mTORC1 requires its relocalization to the lysosomes mediated by the Ragulator complex and the Rag GTPases. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at "Thr-389", which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation. Within mTORC1, AKT1S1 negatively regulates mTOR activity in a manner that is dependent on its phosphorylation state and binding to 14-3-3 proteins. Inhibits RHEB-GTP-dependent mTORC1 activation. Substrate for AKT1 phosphorylation, but can also be activated by AKT1-independent mechanisms. May also play a role in nerve growth factor-mediated neuroprotection.

Molecular Function

negative regulation of cell size, negative regulation of protein kinase activity, negative regulation of TOR signaling, neurotrophin TRK receptor signaling pathway, regulation of cellular response to heat, regulation of neuron apoptotic process

Subcellular Location

Cytoplasm > cytosol

Expression

Widely expressed with highest levels of expression in liver and heart. Expressed at higher levels in cancer cell lines (e.g. A-549 and HeLa) than in normal cell lines (e.g. HEK293).

Alternative Nomenclature

40 kDa proline rich AKT substrate antibody AKT1 S1 antibodyAKT1 substrate 1 (proline rich) antibodyAKT1S1 antibody Lobe antibody PRAS antibodyPRAS40 antibody Proline rich Akt substrate 40 kDa antibodyProline-rich AKT1 substrate 1 antibody