General Information

Product

Prominin 1 Antibody

Verified Applications

ELISA, IHC, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid regions 700-730 on CD133 protein

Accession #

NCBI: XP_006714037.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.50-0.95 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:2,000

ELISA

1:2,000

Western Blot

1:500-1:1,000

Protein

Uniprot #

UPI0003EAF562

Overview

May play a role in cell differentiation, proliferation and apoptosis (PubMed:24556617). Binds cholesterol in cholesterol-containing plasma membrane microdomains and may play a role in the organization of the apical plasma membrane in epithelial cells. During early retinal development acts as a key regulator of disk morphogenesis. Involved in regulation of MAPK and Akt signaling pathways. In neuroblastoma cells suppresses cell differentiation such as neurite outgrowth in a RET-dependent manner (PubMed:20818439).

Molecular Function

Actinin binding; Cadherin binding

Subcellular Location

Apical cell membrane; Multi-pass membrane protein; microvillus membrane; cilium; photoreceptor outer segment; Endoplasmic reticulum-Golgi intermediate compartment

Expression

Isoform 1 is selectively expressed on CD34 hematopoietic stem and progenitor cells in adult and fetal bone marrow, fetal liver, cord blood and adult peripheral blood. Isoform 1 is not detected on other blood cells. Isoform 1 is also expressed in a number of non-lymphoid tissues including retina, pancreas, placenta, kidney, liver, lung, brain and heart. Found in saliva within small membrane particles. Isoform 2 is predominantly expressed in fetal liver, low in bone marrow, and barely detectable in peripheral blood. Isoform 2 is expressed on hematopoietic stem cells and in epidermal basal cells (at protein level). Expressed in adult retina by rod and cone photoreceptor cells (at protein level).

Structure

Interacts with CDHR1 and with actin filaments. Interacts with NAT8 and NAT8B.

Alternative Nomenclature

AC133 antibody

CD133 antibody

CORD12 antibody

Hematopoietic stem cell antigen antibody

MCDR2 antibody

MSTP061 antibody

RP41 antibody

STGD4 antibody