General Information

Product

TSG101 Antibody

Description

Affinity Purified Tumor susceptibility gene 101 protein Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 330-390 on Tumor susceptibility gene 101 protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5-1.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Purification Method

Immobilized antigen affinity chromatography

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunocytochemistry

1:50-1:150

Immunohistochemistry

1:50-1:150

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

Q99816

Overview

Component of the ESCRT-I complex, a regulator of vesicular trafficking process. Binds to ubiquitinated cargo proteins and is required for the sorting of endocytic ubiquitinated cargos into multivesicular bodies (MVBs). Mediates the association between the ESCRT-0 and ESCRT-I complex. Required for completion of cytokinesis; the function requires CEP55. May be involved in cell growth and differentiation. Acts as a negative growth regulator. Involved in the budding of many viruses through an interaction with viral proteins that contain a late-budding motif P-[ST]-A-P. This interaction is essential for viral particle budding of numerous retroviruses. Required for the exosomal release of SDCBP, CD63 and syndecan (PubMed:22660413).

Molecular Function

calcium-dependent protein binding

DNA binding

ligand-dependent nuclear receptor transcription coactivator activity

protein homodimerization activity

transcription corepressor activity

ubiquitin binding

ubiquitin protein ligase binding

virion binding

Subcellular Location

Cytoplasm

Membrane; Peripheral membrane protein

Nucleus

Late endosome membrane; Peripheral membrane protein

Expression

Heart, brain, placenta, lung, liver, skeletal, kidney and pancreas.

Alternative Nomenclature

ESCRT I complex subunit TSG101 antibody

TS101_HUMAN antibody

TSG 10 antibody

TSG 101 antibody

Tumor susceptibility gene 10 antibody

Tumor susceptibility gene 101 antibody

Tumor susceptibility protein antibody

Tumor susceptibility protein isoform 3 antibody

VPS23 antibody