General Information

Product

Legumain Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 100-150 on human Legumain protein.

Accession #

NCBI: XP_016876954

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Purification Method

Immobilized antigen affinity chromatography

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:50,000

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

Q99538

Overview

Has a strict specificity for hydrolysis of asparaginyl bonds. Can also cleave aspartyl bonds slowly, especially under acidic conditions. Required for normal lysosomal protein degradation in renal proximal tubules. Required for normal degradation of internalized EGFR. Plays a role in the regulation of cell proliferation via its role in EGFR degradation. May be involved in the processing of proteins for MHC class II antigen presentation in the lysosomal/endosomal system.

Subcellular Location

Lysosome

Expression

Ubiquitous. Particularly abundant in kidney, heart and placenta.

Structure

In the zymogen form, the uncleaved propeptide blocks access to the active site.

Alternative Nomenclature

AEP antibodyAsparaginyl endopeptidase antibodycysteine 1 antibodyCysteine protease 1 antibody Legumain antibodyLGMN antibodyLGMN1 antibodyProtease antibodyProtease cysteine 1 (legumain) antibodyProtease cysteine 1 antibodyPRSC1 antibody