General Information

Product

Sequestosome-1 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 400-440 on human Sequestosome-1 protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Purification Method

Immobilized antigen affinity chromatography

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

Q13501

Overview

Autophagy receptor that interacts directly with both the cargo to become degraded and an autophagy modifier of the MAP1 LC3 family. Required both for the formation and autophagic degradation of polyubiquitin-containing bodies, called ALIS (aggresome-like induced structures) and links ALIS to the autophagic machinery. Involved in midbody ring degradation. May regulate the activation of NFKB1 by TNF-alpha, nerve growth factor (NGF) and interleukin-1. May play a role in titin/TTN downstream signaling in muscle cells. May regulate signaling cascades through ubiquitination. Adapter that mediates the interaction between TRAF6 and CYLD. May be involved in cell differentiation, apoptosis, immune response and regulation of K+ channels.

Molecular Function

identical protein binding, protein kinase binding, protein kinase C binding, protein serine/threonine kinase activity, receptor tyrosine kinase binding, SH2 domain binding, ubiquitin binding, ubiquitin protein ligase binding, zinc ion binding

Subcellular Location

Cytoplasm

Late endosome

Lysosome

Cytoplasmic vesicle › autophagosome

Nucleus

Endoplasmic reticulum

Cytoplasm › P-body

Expression

Ubiquitously expressed.

Structure

Homooligomer or heterooligomer; may form homotypic arrays. Dimerization interferes with ubiquitin binding.

Alternative Nomenclature

A170

EBI 3 associated protein of 60 kDa

EBIAP

FTDALS3

ORCA

OSF-6

Osi

OSIL

Oxidative stress induced like

p60

p62

p62B

Paget disease of bone 3

PDB3

Phosphotyrosine independent ligand for the Lck SH2 domain of 62 kDa

PKC-zeta-interacting protein

Protein kinase C-zeta-interacting protein

Sequestosome 1

SQSTM 1

STAP

STONE14

Ubiquitin-binding protein p62

ZIP

ZIP3