General Information

Product

B-raf Antibody

Description

Affinity Purified Serine/threonine-protein kinase B-raf Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Monkey, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 700-750 on human serine/threonine-protein kinase B-raf protein.

Accession #

NCBI: NP_004324.2

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunoprecipitation

1:200

Western Blot

1:500. Molecular Weight: 84.4

Protein

Uniprot #

P15056

Overview

Protein kinase involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron. Phosphorylates MAP2K1, and thereby contributes to the MAP kinase signal transduction pathway.

Molecular Function

Kinase, Serine/threonine-protein kinase, Transferase

Subcellular Location

Nucleus, Cytoplasm, Cell membrane

Expression

Brain and testis.

Structure

Monomer. Homodimer. Heterodimerizes with RAF1, and the heterodimer possesses a highly increased kinase activity compared to the respective homodimers or monomers. Heterodimerization is mitogen-regulated and enhanced by 14-3-3 proteins. MAPK1/ERK2 activation can induce a negative feedback that promotes the dissociation of the heterodimer by phosphorylating BRAF at Thr-753. Found in a complex with at least BRAF, HRAS, MAP2K1, MAPK3 and RGS14. Interacts with RIT1. Interacts (via N-terminus) with RGS14 (via RBD domains); the interaction mediates the formation of a ternary complex with RAF1, a ternary complex inhibited by GNAI1. Interacts with DGKH. Interacts with PRMT5. Interacts with KSR2.

Alternative Nomenclature

94 kDa B raf protein antibody

B raf 1 antibody

B Raf proto oncogene serine threonine protein kinase antibody

B RAF1 antibody

B-Raf proto-oncogene serine/threonine-protein kinase (p94) antibody

BRAF 1 antibody

cRmil antibody

Murine sarcoma viral v raf oncogene homolog B1 antibody

NS7 antibody

Oncogen BRAF antibody

p94 antibody

Proto-oncogene B-Raf antibody

Proto-oncogene c-Rmil antibody

RAFB1 antibody

RMIL antibody

Serine/threonine-protein kinase B-raf antibody

v-Raf murine sarcoma viral oncogene homolog B1 antibody