General Information

Product

Topoisomerase II alpha Antibody

Description

Affinity Purified DNA topoisomerase (ATP-hydrolysing) Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 1220-1270 on human DNA topoisomerase (ATP-hydrolysing) protein.

Accession #

GenBank: CAA09762.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.54-0.57 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

P11388

Overview

Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks. Essential during mitosis and meiosis for proper segregation of daughter chromosomes.

Molecular Function

Isomerase, Topoisomerase, ATP binding

Subcellular Location

Cytoplasm, Nucleus

Structure

Homodimer. Interacts with COPS5. Interacts with RECQL5; this stimulates DNA decatenation. Interacts with SETMAR; stimulates the topoisomerase activity.

Alternative Nomenclature

alpha isozyme antibody

ATP hydrolyzing DNA topoisomerase II alfa antibody

DNA gyrase antibody

DNA topoisomerase 2 alpha antibody

DNA topoisomerase II 170 kD antibody

DNA topoisomerase II alpha isozyme antibody

TOP2 antibody

TOP2A antibody

Topoisomerase DNA II alpha 170kDa antibody

TP2A antibody

Synthetic peptide taken within amino acid region 1220-1270 on human DNA topoisomerase (ATP-hydrolysing) protein.