General Information

Product

ATG16L1 Antibody

Description

Affinity Purified Autophagy-related protein 16-1 Antibody

Verified Applications

ELISA, IHC,IP, WB

Host

Rabbit

Species Cross Reactivity

Mouse, Rat

Predicted: Human

Immunogen

Synthetic peptide taken within amino acid region 560-610 on human Autophagy-related protein 16-1.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Purification Method

Immobilized antigen affinity chromatography

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

Q676U5

Overview

Plays an essential role in autophagy: interacts with ATG12-ATG5 to mediate the conjugation of phosphatidylethanolamine (PE) to LC3 (MAP1LC3A, MAP1LC3B or MAP1LC3C), to produce a membrane-bound activated form of LC3 named LC3-II. Thereby, controls the elongation of the nascent autophagosomal membrane.

Molecular Function

Identical protein binding, Ubiquitin-like protein transferase activity

Subcellular Location

Cytoplasm, Preautophagosomal structure membrane, Peripheral membrane protein

Structure

Homooligomer. Interacts with WIPI2, FIP200 and ATG5. Part of either the minor and major complexes respectively composed of 4 sets of ATG12-ATG5 and ATG16L1 (400 kDa) or 8 sets of ATG12-ATG5 and ATG16L1 (800 kDa). Interacts with RAB33B. Interacts with TMEM

Alternative Nomenclature

APG16 like 1 antibody

APG16L antibody

APG16L beta antibody

ATG16 autophagy related 16 like 1 antibody

ATG16A antibody

ATG16L antibody

Autophagy related protein 16 1 antibody

IBD10 antibody

WD repeat domain 30 antibody

WDR30 antibody