General Information

Product

iNOS Antibody

Description

Affinity Purified Nitric oxide synthase, inducible Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 100-150 on human inducible nitric oxide synthase protein.

Specificity

This antibody will detect both iNOS and iNOS2 protein.

Accession #

GenBank: BAA37123.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.75 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

P35228

Overview

Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such COX2.

Molecular Function

Arginine binding, FMN binding, iron ion binding, NADPH-hemoprotein reductase activity, nitric-oxide synthase activity, receptor binding, heme binding, NADP binding

Subcellular Location

cortical cytoskeleton; cytoplasm; cytosol; intracellular; nucleus; perinuclear region of cytoplasm; peroxisome

Expression

Expressed in the liver, retina, bone cells and airway epithelial cells of the lung. Not expressed in the platelets.

Structure

Belongs to the NOS family.

Contains 1 FAD-binding FR-type domain.

Contains 1 flavodoxin-like domain.

Alternative Nomenclature

HEP NOS

Hepatocyte NOS

Inducible nitric oxide synthase

Inducible NO synthase

INOS

MAC NOS

Macrophage NOS

Nitric oxide synthase 2 inducible macrophage

Nitric oxide synthase

NOS 2

NOS

NOS2A

NOS2A, Inducible, Hepatocyte

Peptidyl-cysteine S-nitrosylase NOS2