General Information

Product

Progesterone Receptor Antibody

Description

Affinity Purified Progesterone Receptor Antibody N-epitope

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Cat, Human, Monkey, Pig

Immunogen

Synthetic peptide taken within amino acid region 320-370 on human Progesterone receptor protein.

Accession #

GenBank: AAD01587.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.50 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

N-epitope

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

P06401

Overview

The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Progesterone receptor isoform B (PRB) is involved activation of c-SRC/MAPK signaling on hormone stimulation.Isoform A: inactive in stimulating c-Src/MAPK signaling on hormone stimulation.Isoform 4: Increases mitochondrial membrane potential and cellular respiration upon stimulation by progesterone.

Molecular Function

DNA binding; Enzyme binding; RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription; Steriod hormone receptor activity; Transcription regulation

Subcellular Location

Nucleus. CytoplasmNote: Nucleoplasmic shuttling is both homone- and cell cycle-dependent. On hormone stimulation, retained in the cytoplasm in the G1 and G2/M phases.Isoform A : Nucleus. CytoplasmNote: Mainly nuclear.Isoform 4 : Mitochondrion outer membrane mitochondrial outer membrane Cytoplasm, Membrane, Mitochondrion, Mitochondrion outer membrane, Nucleus

Structure

Interacts with SMARD1 and UNC45A. Interacts with CUEDC2; the interaction promotes ubiquitination, decreases sumoylation, and repesses transcriptional activity. Interacts with PIAS3; the interaction promotes sumoylation of PR in a hormone-dependent manner, inhibits DNA-binding, and alters nuclear export. Interacts with SP1; the interaction requires ligand-induced phosphorylation on Ser-345 by ERK1/2 MAPK. Interacts with PRMT2.

Alternative Nomenclature

NR3C3 antibody

Nuclear receptor subfamily 3 group C member 3 antibody

PGR antibody

PR antibody

PR antibody

PRA antibody

PRB antibody

Progesterone receptor antibody

Progestin receptor form A antibody

Progestin receptor form B antibody

Progestin receptor form C antibody