General Information

Product

SIRT5 Antibody

Description

Affinity Purified Sirtuin 5 (silent mating type information regulation 2 homolog) 5 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Chicken, Human, Monkey, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 260-310on rat Sirtuin 5 protein.

Accession #

NCBI:NP_001004256.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.66-0.68 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

Q68FX9

Overview

NAD-dependent lysine demalonylase and desuccinylase that specifically removes malonyl and succinyl groups on target proteins. Activates CPS1 and contributes to the regulation of blood ammonia levels during prolonged fasting: acts by mediating desuccinylation of CPS1, thereby increasing CPS1 activity in response to elevated NAD levels during fasting. Activates SOD1 by mediating its desuccinylation, leading to reduced reactive oxygen species. Has weak NAD-dependent protein deacetylase activity; however this activity may not be physiologically relevant in vivo. Can deacetylate cytochrome c (CYCS) and a number of other proteins in vitro.

Molecular Function

Hydrolase; Metal-binding; NAD; Zinc

Subcellular Location

Isoform 1: Cytoplasm, Mitochondrion Isoform 2: Mitochondrion

Expression

Widely expressed.

Structure

Interacts with CPS1 (By similarity). Monomer. Homodimer. Forms homodimers upon suramin binding.

Alternative Nomenclature

NAD dependent deacetylase sirtuin 5 antibody

Regulatory protein SIR2 homolog 5 antibody

Silent mating type information regulation 2 Sir2 like 5 antibody

SIR2-like protein 5 antibody

SIR2L5 antibody

Sirtuin type 5 antibody